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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia; Embrapa Amazônia Oriental. |
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Data corrente: |
18/10/2011 |
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Data da última atualização: |
21/10/2011 |
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Autoria: |
COLLARES, D. G.; LIMA, A. L. S. de. |
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Afiliação: |
DANIELA GARCIA COLLARES, CNPAE; ANA LAURA SILVA DE LIMA, CPATU. |
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Título: |
Programa capacita multiplicadores na cultura de dendê: Embrapa e parceiros promovem capacitação em diversos aspectos da cultura da palma de óleo. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Agroenergia em revista, ano 2, n. 2, p. 32, maio 2011. |
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Idioma: |
Português |
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Palavras-Chave: |
Embrapa e parceiros; Palma de óleo. |
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Thesagro: |
Capacitação; Dendê. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/44258/1/Revista-Agroenergia-2-1416.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/67352/1/Revista-Agroenergia-2-1416.pdf
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Marc: |
LEADER 00566naa a2200169 a 4500
001 1903294
005 2011-10-21
008 2011 bl uuuu u00u1 u #d
100 1 $aCOLLARES, D. G.
245 $aPrograma capacita multiplicadores na cultura de dendê$bEmbrapa e parceiros promovem capacitação em diversos aspectos da cultura da palma de óleo.
260 $c2011
650 $aCapacitação
650 $aDendê
653 $aEmbrapa e parceiros
653 $aPalma de óleo
700 1 $aLIMA, A. L. S. de
773 $tAgroenergia em revista, ano 2$gn. 2, p. 32, maio 2011.
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Registro original: |
Embrapa Agroenergia (CNPAE) |
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
03/08/2017 |
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Data da última atualização: |
29/12/2017 |
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Autoria: |
MENEGHEL, M.; DALL'ACQUA, P. C.; AMBROGI, M.; LEÃO, B. C. S.; ROCHA-FRIGONI, N. A. S.; MINGOTI, G. Z. |
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Afiliação: |
Melissa Meneghel, Universidade Estadual Paulista-UNESP/Programa de Pós-Graduação em Medicina Veterinária (Reprodução Animal)/Faculdade de Ciências Agrárias e Veterinárias; Priscila Chediek Dall’Acqua, Universidade Estadual Paulista-UNESP/Programa de Pós-Graduação em Medicina Veterinária (Reprodução Animal)/Faculdade de Ciências Agrárias e Veterinárias; Marcela Ambrogi, Universidade Estadual Paulista-UNESP/Programa de Pós-Graduação em Medicina Veterinária (Reprodução Animal)/Faculdade de Ciências Agrárias e Veterinárias; Beatriz C. S. Leão, Universidade Estadual Paulista-UNESP/Programa de Pós-Graduação em Medicina Veterinária (Reprodução Animal)/Faculdade de Ciências Agrárias e Veterinárias; Nathália A. S. Rocha-Frigoni, Universidade Estadual Paulista-UNESP/Programa de Pós-Graduação em Medicina Veterinária (Reprodução Animal)/Faculdade de Ciências Agrárias e Veterinárias; Gisele Z. Mingoti, Universidade Estadual Paulista-UNESP/Programa de Pós-Graduação em Medicina Veterinária (Reprodução Animal)/Faculdade de Ciências Agrárias e Veterinárias. |
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Título: |
Lipid content and cryotolerance of in vitro-produced bovine embryos treated with forskolin before vitrification. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n. 4, p. 395-400, abril 2017. |
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Idioma: |
Inglês |
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Notas: |
Título em português: Acúmulo de lipídios intracitoplasmáticos, desenvolvimento e criotolerância de embriões bovinos produzidos in vitro e tratados com diferentes concentrações de forskolin antes da vitrificação. |
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Conteúdo: |
The aim of the present study was to evaluate the intracytoplasmic lipid content, development and cryotolerance of in vitro-produced bovine embryos treated with different concentrations of forskolin before vitrification. Embryos were produced from abattoir-derived ovaries and allocated into four groups. In the treatment groups, forskolin was added to the in vitro culture medium on Day 6 and incubated for 24 hours in one of the following concentrations: 2.5μM (Forsk 2.5 group), 5.0μM (Forsk 5.0 group) or 10.0μM (Forsk 10.0 group). Embryos from the control group were cultured without forskolin. On Day 7 of culture, the expanded blastocysts were stained with the lipophilic dye Sudan Black B for determination of the intracytoplasmic lipid content or were cryopreserved via the Vitri- -Ingá® procedure. Although there were no significant differences (P>0.05) in the blastocyst rates between the Control group (44.9%) and the other treatments, the embryo production was lower (P<0.05) in Forsk 10.0 group (38.8%) compared to Forsk 2.5 (50.5%) and Forsk 5.0 (54.7%) groups. The intracytoplasmic lipid content (expressed in arbitrary units of pixels) in blastocysts from the Control group (1.00±0.03) was similar (P>0.05) to that found in Forsk 2.5 (0.92±0.03) and Forsk 10.0 groups (1.06±0.03) groups; however the lipid accumulation in blastocysts from Forsk 5.0 group (0.82±0.04) was lower than in the Control group (P<0.05). Based on these results, Forsk 5.0 treatment was tested for cryotolerance and it was observed that the blastocoel re-expansion rate evaluated 24 hours after warming was greater (P<0.05) in Forsk 5.0 group (72.2%) compared to the Control group (46.2%). In conclusion, pre-treatment with forskolin at a concentration of 5.0 μM for 24 hours before vitrification is effective in reducing the intracytoplasmic lipid content and, consequently, improves cryotolerance of IVP bovine embryos. MenosThe aim of the present study was to evaluate the intracytoplasmic lipid content, development and cryotolerance of in vitro-produced bovine embryos treated with different concentrations of forskolin before vitrification. Embryos were produced from abattoir-derived ovaries and allocated into four groups. In the treatment groups, forskolin was added to the in vitro culture medium on Day 6 and incubated for 24 hours in one of the following concentrations: 2.5μM (Forsk 2.5 group), 5.0μM (Forsk 5.0 group) or 10.0μM (Forsk 10.0 group). Embryos from the control group were cultured without forskolin. On Day 7 of culture, the expanded blastocysts were stained with the lipophilic dye Sudan Black B for determination of the intracytoplasmic lipid content or were cryopreserved via the Vitri- -Ingá® procedure. Although there were no significant differences (P>0.05) in the blastocyst rates between the Control group (44.9%) and the other treatments, the embryo production was lower (P<0.05) in Forsk 10.0 group (38.8%) compared to Forsk 2.5 (50.5%) and Forsk 5.0 (54.7%) groups. The intracytoplasmic lipid content (expressed in arbitrary units of pixels) in blastocysts from the Control group (1.00±0.03) was similar (P>0.05) to that found in Forsk 2.5 (0.92±0.03) and Forsk 10.0 groups (1.06±0.03) groups; however the lipid accumulation in blastocysts from Forsk 5.0 group (0.82±0.04) was lower than in the Control group (P<0.05). Based on these results, Forsk 5.0 treatment was tested ... Mostrar Tudo |
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Palavras-Chave: |
Acúmulo lipídico intracitoplasmático; Criotolerância; Lipídio intracitoplasmático; Produção in vitro; Vitrificação. |
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Thesagro: |
Bovino; Embrião. |
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Thesaurus NAL: |
forskolin. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/162413/1/Lipid-content-and-cryotolerance.pdf
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Marc: |
LEADER 03070naa a2200289 a 4500
001 2073597
005 2017-12-29
008 2017 bl uuuu u00u1 u #d
100 1 $aMENEGHEL, M.
245 $aLipid content and cryotolerance of in vitro-produced bovine embryos treated with forskolin before vitrification.$h[electronic resource]
260 $c2017
500 $aTítulo em português: Acúmulo de lipídios intracitoplasmáticos, desenvolvimento e criotolerância de embriões bovinos produzidos in vitro e tratados com diferentes concentrações de forskolin antes da vitrificação.
520 $aThe aim of the present study was to evaluate the intracytoplasmic lipid content, development and cryotolerance of in vitro-produced bovine embryos treated with different concentrations of forskolin before vitrification. Embryos were produced from abattoir-derived ovaries and allocated into four groups. In the treatment groups, forskolin was added to the in vitro culture medium on Day 6 and incubated for 24 hours in one of the following concentrations: 2.5μM (Forsk 2.5 group), 5.0μM (Forsk 5.0 group) or 10.0μM (Forsk 10.0 group). Embryos from the control group were cultured without forskolin. On Day 7 of culture, the expanded blastocysts were stained with the lipophilic dye Sudan Black B for determination of the intracytoplasmic lipid content or were cryopreserved via the Vitri- -Ingá® procedure. Although there were no significant differences (P>0.05) in the blastocyst rates between the Control group (44.9%) and the other treatments, the embryo production was lower (P<0.05) in Forsk 10.0 group (38.8%) compared to Forsk 2.5 (50.5%) and Forsk 5.0 (54.7%) groups. The intracytoplasmic lipid content (expressed in arbitrary units of pixels) in blastocysts from the Control group (1.00±0.03) was similar (P>0.05) to that found in Forsk 2.5 (0.92±0.03) and Forsk 10.0 groups (1.06±0.03) groups; however the lipid accumulation in blastocysts from Forsk 5.0 group (0.82±0.04) was lower than in the Control group (P<0.05). Based on these results, Forsk 5.0 treatment was tested for cryotolerance and it was observed that the blastocoel re-expansion rate evaluated 24 hours after warming was greater (P<0.05) in Forsk 5.0 group (72.2%) compared to the Control group (46.2%). In conclusion, pre-treatment with forskolin at a concentration of 5.0 μM for 24 hours before vitrification is effective in reducing the intracytoplasmic lipid content and, consequently, improves cryotolerance of IVP bovine embryos.
650 $aforskolin
650 $aBovino
650 $aEmbrião
653 $aAcúmulo lipídico intracitoplasmático
653 $aCriotolerância
653 $aLipídio intracitoplasmático
653 $aProdução in vitro
653 $aVitrificação
700 1 $aDALL'ACQUA, P. C.
700 1 $aAMBROGI, M.
700 1 $aLEÃO, B. C. S.
700 1 $aROCHA-FRIGONI, N. A. S.
700 1 $aMINGOTI, G. Z.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 37, n. 4, p. 395-400, abril 2017.
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